|
KMID : 0613820020120060803
|
|
Journal of Life Science
2002 Volume.12 No. 6 p.803 ~ p.811
|
|
|
Antimicrobial activity of the hexane extract of Stachys sieboldii MIQ leaf
|
|
Ryu Beung-Ho
Park Bub-Gu
|
|
|
Abstract
|
|
|
|
The present study was carried out for research and development of natural antimicrobial from extract of Stachys sieboldii MIQ against food borne bacteria. The hexane extract of Stachys sieboldii MIQ at 250㎍/mL per disc showed 15～20 mm inhibition zone against gram positive and gram negative bacteria. Minimum inhibitory concentration (MIC) of hexane extract was 250㎍/mL against Bacillus cereus, 250~500㎍/mL against Listeria monocytogenes, 500㎍/mL against Staphylococcus aureus, Psedomonas aeruginosa. Observation by transmission electron microscope, showed that disruption of the cell wall assumed to be due to the bactericidal activity. In addition, the membrane integrity of the sensitive cells was disrupted by exposure to Stachys sieboldii MIQ extract on the D-β-galatosidase activity as substrate of O-nitrophenol-β-D-galacto-pyranoside. The hexane extract of Stachys sieboldii MIQ was very stable on the pH and thermal stability.
|
|
|
KEYWORD
|
|
|
Stachys sieboldii., antimicrobial activity, bacteria
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|